https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38846 Raphidiopsis raciborskii is an invasive bloom-forming cyanobacteria with the flexibility to utilize atmospheric and fixed nitrogen. Since nitrogen-fixation has a high requirement for iron as an ezyme cofactor, we hypothesize that iron availability would determine the success of the species under nitrogen-fixing conditions. This study compares the proteomic response of cylindrospermopsin-producing and non-toxic strains of R. racibroskii to reduced iron concentrations, under nitrogen-fixing conditions, to examine any strain-specific adaptations that might increase fitness under these conditions. We also compared their proteomic responses at exponential and stationary growth phases to capture the changes throughout the growth cycle. Overall, the toxic strain was more competitive under Fe-starved conditions during exponential phase, with upregulated growth and transport-related proteins. The non-toxic strain showed reduced protein expression across multiple primary metabolism pathways. We propose that the increased expression of porin proteins during the exponential growth phase enables toxic strains to persist under Fe-starved conditions with this ability providing a potential explanation for the increased fitness of cylindrospermoipsin-producing strains during unfavourable environmental conditions.]]> Mon 06 May 2024 13:15:09 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:35132 Raphidiopsis raciborskii is of environmental and social concern in view of its toxicity, bloom‐forming characteristics and increasingly widespread occurrence. However, while availability of macronutrients and micronutrients such as N and Fe are critically important for the growth and metabolism of this organism, the physiological response of toxic and non‐toxic strains of R. raciborskii to varying Fe and N availabilities remains unclear. By determining physiological parameters as a function of Fe and N availability, we demonstrate that R. raciborskii growth and N₂‐fixing activity are facilitated at higher Fe availability under N₂‐limited conditions with faster growth of the CS‐506 (cylindrospermopsin‐producing) strain compared with that of CS‐509 (the non‐toxic) strain. Radiolabelled Fe uptake assays indicated that R. raciborskii acclimated under Fe‐limited conditions acquires Fe at significantly higher rates than under Fe replete conditions, principally via unchelated Fe(II) generated as a result of photoreduction of complexed Fe(III). While N₂‐fixation of both strains occurred during both day and night, the CS‐506 strain overall exhibited higher N₂‐fixing and Fe uptake rates than the CS‐509 strain under N‐deficient and Fe‐limited conditions. The findings of this study highlight that Fe availability is of significance for the ecological advantage of CS‐506 over CS‐509 in N‐deficient freshwaters.]]> Fri 03 Apr 2020 12:05:38 AEDT ]]>